Jump to main content
Jump to site search

Issue 3, 2007
Previous Article Next Article

Polymer-tethered membranes as quantitative models for the study of integrin-mediated cell adhesion

Author affiliations

Abstract

Here we report a remarkable enhancement in the adhesion strength of transmembrane cell receptors, human platelet integrin, in a new class of supported lipid membranes, which are separated from the solid substrates by linear polymer spacers. The amphiphilic polymer tether consists of linear hydrophilic poly(2-oxazoline) chains of defined length (degree of polymerizationn = 104, MW/Mn = 1.30), whose chain termini are functionalized with the tri-functional silane surface coupling group and hydrophobic n-alkyl chains as membrane anchors (lipopolymers). As a model of test cells, giant lipid vesicles were functionalized with synthetic ligand molecules containing the RGD sequence, and the free energy of adhesion Δgad between the integrin-doped tethered membrane and the vesicle was measured using a micro-interferometry technique. It has been demonstrated that the adhesion function of integrin receptors in these polymer-tethered membranes is 30 times stronger than those incorporated into membranes directly deposited onto solid substrates (solid-supported membranes). The obtained results demonstrate that linear lipopolymer spacers provide a fluid and non-denaturing environment for the incorporated cell receptors and allow quantitative modelling of cell adhesion processes.

Graphical abstract: Polymer-tethered membranes as quantitative models for the study of integrin-mediated cell adhesion

Back to tab navigation

Publication details

The article was received on 22 Aug 2006, accepted on 27 Oct 2006 and first published on 23 Nov 2006


Article type: Paper
DOI: 10.1039/B612069E
Soft Matter, 2007,3, 333-336

  •   Request permissions

    Polymer-tethered membranes as quantitative models for the study of integrin-mediated cell adhesion

    O. Purrucker, S. Gönnenwein, A. Förtig, R. Jordan, M. Rusp, M. Bärmann, L. Moroder, E. Sackmann and M. Tanaka, Soft Matter, 2007, 3, 333
    DOI: 10.1039/B612069E

Search articles by author

Spotlight

Advertisements