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Issue 13, 2007
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The role of metal ions in phosphate ester hydrolysis

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Abstract

Many phosphatases make use of metal ions to aid catalysis of phosphate ester hydrolysis. Here, we investigate the impact of metal ions on the potential energy surface (PES), and hence the preferred reaction mechanism, for a simple model for hydrolysis of phosphate ester monoanions. We show that, while both associative (AN + DN) and dissociative (DN + AN) mechanisms are represented on the potential energy surfaces both in the presence and absence of metal ions, the DN + AN process is favoured when there are no metal ions present and the AN + DN process is favoured in the presence of two metal ions. A concerted (ANDN) process is also available in the presence of two metal ions, but proceeds via a high-energy transition state. In the presence of only a single metal ion the ANDN process is the most favoured, but still proceeds via a high-energy transition state. Thus, we conclude that metallo-enzyme phosphatases are likely to utilise an associative process, while those that function without metal ions may well follow a dissociative process.

Graphical abstract: The role of metal ions in phosphate ester hydrolysis

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Publication details

The article was received on 29 Jan 2007, accepted on 04 May 2007 and first published on 30 May 2007


Article type: Paper
DOI: 10.1039/B701274H
Citation: Org. Biomol. Chem., 2007,5, 2098-2108
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    The role of metal ions in phosphate ester hydrolysis

    S. C. L. Kamerlin and J. Wilkie, Org. Biomol. Chem., 2007, 5, 2098
    DOI: 10.1039/B701274H

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