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Issue 8, 2007
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Optical filtering technologies for integrated fluorescence sensors

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Numerous approaches have been taken to miniaturizing fluorescence sensing, which is a key capability for micro-total-analysis systems. This critical, comprehensive review focuses on the optical hardware required to attenuate excitation light while transmitting fluorescence. It summarizes, evaluates, and compares the various technologies, including filtering approaches such as interference filters and absorption filters and filterless approaches such as multicolor sensors and light-guiding elements. It presents the physical principles behind the different architectures, the state-of-the-art micro-fluorometers and how they were microfabricated, and their performance metrics. Promising technologies that have not yet been integrated are also described. This information will permit the identification of methods that meet particular design requirements, from both performance and integration perspectives, and the recognition of the remaining technological challenges. Finally, a set of performance metrics are proposed for evaluating and reporting spectral discrimination characteristics of integrated devices in order to promote side-by-side comparisons among diverse technologies and, ultimately, to facilitate optimized designs of micro-fluorometers for specific applications.

Graphical abstract: Optical filtering technologies for integrated fluorescence sensors

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Publication details

The article was received on 19 Mar 2007, accepted on 13 Jun 2007 and first published on 10 Jul 2007

Article type: Critical Review
DOI: 10.1039/B704008C
Lab Chip, 2007,7, 955-977

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    Optical filtering technologies for integrated fluorescence sensors

    M. Dandin, P. Abshire and E. Smela, Lab Chip, 2007, 7, 955
    DOI: 10.1039/B704008C

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