Issue 9, 2007
From the journal:

Journal of Analytical Atomic Spectrometry

Combining UHR-SEC-HPLC-ICP-MS with flow cytometry to quantify metallothioneins and to study zinc homeostasis in human PBMC†‡

Marco Malavolta,*a   Francesco Piacenza,a   Laura Costarelli,a   Robertina Giacconi,a   Elisa Muti,a   Catia Cipriano,a   Silvia Tesei,a   Sandro Speziab  and  Eugenio Mocchegiania  

* Corresponding authors

a Immunology Ctr. (Sect. Nutrition, Immunity and Ageing) Res. Dept. INRCA, via Birarelli 8, Ancona, Italy
E-mail: m.malavota@inrca.it
Fax: +39 071 206791
Tel: +39 071 8004116

b Thermo Fisher Scientific, Strada Rivoltana, Rodano (MI), Italy
E-mail: sandro.spezia@thermofisher.com
Fax: +39 02 95059426
Tel: +39 02 95059319

Abstract

A simple ultra high resolution (UHR)-size exclusion HPLC (SEC-HPLC) system with online detection by ICP-MS was set-up to quantify metallothioneins (MT), Zn bound to MT and to other cytosolic protein fractions with defined molecular weight in human peripheral blood mononuclear cells (PBMC). Applications of this method in combination with flow cytometry (FC) offer powerful diagnostic approaches for the evaluation of the zinc status and for quantifying MT in a very small sample amount.

Graphical abstract: Combining UHR-SEC-HPLC-ICP-MS with flow cytometry to quantify metallothioneins and to study zinc homeostasis in human PBMC

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Article information

DOI
https://doi.org/10.1039/B704577H
Article type
Technical Note
Submitted
28 Mar 2007
Accepted
07 Jun 2007
First published
26 Jun 2007

J. Anal. At. Spectrom., 2007,22, 1193-1198

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Combining UHR-SEC-HPLC-ICP-MS with flow cytometry to quantify metallothioneins and to study zinc homeostasis in human PBMC

M. Malavolta, F. Piacenza, L. Costarelli, R. Giacconi, E. Muti, C. Cipriano, S. Tesei, S. Spezia and E. Mocchegiani, J. Anal. At. Spectrom., 2007, 22, 1193 DOI: 10.1039/B704577H

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