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Issue 6, 2007
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Cell motility on micropatterned treadmills and tracks

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Abstract

Surfaces micropatterned with disjointed cell adhesive/non-adhesive regions allow for precise control of cell shape, internal organization and function. In particular, substrates prepared by the reaction–diffusion ASoMic (Anisotropic Solid Microetching) method localize cells onto transparent micro-islands or tracks surrounded by an opaque, adhesion-resistant background. ASoMic is compatible with several important imaging modalities (e.g. wide-field, fluorescent, TIRF and confocal microscopies), and can be used to study and quantify various intracellular and cellular processes related to cell motility. For cells constrained on the islands, the imposed geometry controls spatial organization of the cytoskeleton, while the transparency of the islands allows for real-time analysis of cytoskeletal dynamics. For cells on transparent, linear tracks, the high optical contrast between these adhesive regions and the surrounding non-adhesive background allows for straightforward quantification of the key parameters describing cell motility. Both types of systems provide analytical-quality data that can assist fundamental studies of cell locomotion and can provide a technological basis for cell motility microassays.

Graphical abstract: Cell motility on micropatterned treadmills and tracks

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Publication details

The article was received on 27 Nov 2006, accepted on 27 Feb 2007 and first published on 28 Mar 2007


Article type: Highlight
DOI: 10.1039/B617308J
Soft Matter, 2007,3, 672-679

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    Cell motility on micropatterned treadmills and tracks

    K. Kandere-Grzybowska, C. J. Campbell, G. Mahmud, Y. Komarova, S. Soh and B. A. Grzybowski, Soft Matter, 2007, 3, 672
    DOI: 10.1039/B617308J

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