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Issue 8, 2006
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Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis

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Abstract

The ability to separate living cells is an essential aspect of cell research. Magnetic cell separation methods are among some of the most efficient methods for bulk cell separation. With the development of microfluidic platforms within the biotechnology sector, the design of miniaturised magnetic cell sorters is desirable. Here, we report the continuous sorting of cells loaded with magnetic nanoparticles in a microfluidic magnetic separation device. Cells were passed through a microfluidic chamber and were deflected from the direction of flow by means of a magnetic field. Two types of cells were studied, mouse macrophages and human ovarian cancer cells (HeLa cells). The deflection was dependent on the magnetic moment and size of the cells as well as on the applied flow rate. The experimentally observed deflection matched well with calculations. Furthermore, the separation of magnetic and non-magnetic cells was demonstrated using the same microfluidic device.

Graphical abstract: Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis

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Supplementary files

Article information


Submitted
29 Mar 2006
Accepted
13 Jun 2006
First published
03 Jul 2006

Lab Chip, 2006,6, 974-980
Article type
Paper

Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis

N. Pamme and C. Wilhelm, Lab Chip, 2006, 6, 974
DOI: 10.1039/B604542A

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