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Issue 4, 2006
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Methods to electrophoretically stretch DNA: microcontractions, gels, and hybrid gel-microcontraction devices

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Abstract

The ability to controllably and continuously stretch large DNA molecules in a microfluidic format is important for gene mapping technologies such as Direct Linear Analysis (DLA). We have recently shown that electric field gradients can be readily generated in a microfluidic device and the resulting field is purely elongational. We present a single molecule fluorescence microscopy analysis of T4 DNA (169 kbp) stretching in the electric field gradients in a hyperbolic contraction microchannel. In addition, we are able to selectively pattern a crosslinked gel anywhere inside the microchannel. With an applied electric field, DNA molecules are forced to reptate through the gel and they moderately stretch as they exit the gel. By placing a gel immediately in front of the hyperbolic contraction, we bypass “molecular individualism” and achieve highly uniform and complete stretching of T4 DNA.

Graphical abstract: Methods to electrophoretically stretch DNA: microcontractions, gels, and hybrid gel-microcontraction devices

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Article information


Submitted
28 Oct 2005
Accepted
14 Feb 2006
First published
07 Mar 2006

Lab Chip, 2006,6, 516-525
Article type
Paper

Methods to electrophoretically stretch DNA: microcontractions, gels, and hybrid gel-microcontraction devices

G. C. Randall, K. M. Schultz and P. S. Doyle, Lab Chip, 2006, 6, 516
DOI: 10.1039/B515326C

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