Jump to main content
Jump to site search

Issue 11, 2006
Previous Article Next Article

Subcritical water extraction and β-glucosidase-catalyzed hydrolysis of quercetin glycosides in onion waste

Author affiliations

Abstract

Onion waste is a renewable raw material, rich in different molecular species of the antioxidant quercetin. To utilize this resource, an environmentally sustainable procedure has been developed, using pressurized hot water to extract the quercetin species, followed by biocatalytic conversion of the quercetin glycosides to quercetin and carbohydrates. Two different recombinantly expressed thermostable β-glucosidases, Thermotoga neapolitana β-glucosidase A and B, were utilized as catalysts. These enzymes maintain activity at temperatures around 90 °C, and are therefore ideal to use in combination with hot water extraction. Our results, based on experimental design, showed that they converted quercetin glycosides to active quercetin in less than 10 min reaction time in water at 90 °C, pH 5.0. Experimental design showed that the optimal extraction conditions included three 5 min extraction cycles with water at 120 °C and 50 bars, giving a total extraction time of 15 min. Several different types of quercetin and isorhamnetin glycosides as well as kaempferol were detected in onion waste using LC-MS/MS analysis. After converting the different glycosidic compounds to their respective aglycones, the quercetin content was 10 to 50 mg g−1 dry weight of onion waste (RSD 8%). In summary, our research demonstrates that subcritical water extraction followed by β-glucosidase-catalyzed hydrolysis is a rapid method to determine the content of quercetin and isorhamnetin in onion samples, and is environmentally sustainable as it only uses water as solvent and enzymes as catalysts.

Graphical abstract: Subcritical water extraction and β-glucosidase-catalyzed hydrolysis of quercetin glycosides in onion waste

Back to tab navigation

Publication details

The article was received on 06 Jun 2006, accepted on 10 Aug 2006 and first published on 31 Aug 2006


Article type: Paper
DOI: 10.1039/B608011A
Citation: Green Chem., 2006,8, 949-959

  •   Request permissions

    Subcritical water extraction and β-glucosidase-catalyzed hydrolysis of quercetin glycosides in onion waste

    C. Turner, P. Turner, G. Jacobson, K. Almgren, M. Waldebäck, P. Sjöberg, E. N. Karlsson and K. E. Markides, Green Chem., 2006, 8, 949
    DOI: 10.1039/B608011A

Search articles by author

Spotlight

Advertisements