Sensitive and rapid electrochemical bioassay of glycosidase activity

Tanin Tangkuaram; Jared Q. Gerlach; Yun Xiang; Abdel-Nasser Kawde; Zong Dai; Veer P. Bhavanandan; Jeffrey T. La Belle; Waret Veerasai; Lokesh Joshi; Joseph Wang

doi:10.1039/B605943K

Sensitive and rapid electrochemical bioassay of glycosidase activity

Tanin Tangkuaram,^ab Jared Q. Gerlach,^a Yun Xiang,^a Abdel-Nasser Kawde,^a Zong Dai,^a Veer P. Bhavanandan,^a Jeffrey T. La Belle,^a Waret Veerasai,^b Lokesh Joshi*^a and Joseph Wang*^a

Author affiliations

* Corresponding authors

^a The Biodesign Institute and Fulton School of Engineering, Arizona State University, Tempe, AZ 85287-5801
E-mail: lokesh.joshi@asu.edu, joseph.wang@asu.edu

^b Department of Chemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand

Abstract

Here we present a highly sensitive, rapid and simple electrochemical assay for glycosidases based on treatment of the glycosidase with the appropriate p-nitrophenyl glycoside and anodic detection of released p-nitrophenol. The attractive characteristics of the new bioassay should facilitate advanced glycomic research and routine clinical diagnostics since glycosidases are associated with various diseases.

Analyst

Sensitive and rapid electrochemical bioassay of glycosidase activity

Abstract

Article information

Download Citation

Permissions

Sensitive and rapid electrochemical bioassay of glycosidase activity

Social activity

Search articles by author

Spotlight

Advertisements