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Issue 23, 2006
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Discovery of G-quadruplex stabilizing ligands through direct ELISA of a one-bead-one-compound library

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Abstract

We describe the identification of small-molecule G-quadruplex ligands using a direct ELISA screen of a one-bead-one-compound library of unnatural polyamides displayed on a branched linker with a biotin tag. This general purpose parallel screen for small molecule–oligonucleotide interactions was validated by surface plasmon resonance and ELISA of resynthesized compounds. Linear polyamides displayed similar rankings in their affinity for quadruplex as their branched counterparts. Quadruplex affinity as judged by these surface based techniques was a useful predictor of the ability of the ligands to stabilize the quadruplex to thermal unfolding in solution.

Graphical abstract: Discovery of G-quadruplex stabilizing ligands through direct ELISA of a one-bead-one-compound library

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Supplementary files

Article information


Submitted
14 Aug 2006
Accepted
12 Oct 2006
First published
24 Oct 2006

Org. Biomol. Chem., 2006,4, 4364-4369
Article type
Paper

Discovery of G-quadruplex stabilizing ligands through direct ELISA of a one-bead-one-compound library

J. E. Redman, S. Ladame, A. P. Reszka, S. Neidle and S. Balasubramanian, Org. Biomol. Chem., 2006, 4, 4364
DOI: 10.1039/B611716C

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