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Issue 2, 2006
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Simple SNP typing assay using a base-discriminating fluorescent probe

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Abstract

We have developed a new concept involving a single-step homogeneous method for single-nucleotide polymorphism (SNP) typing. In this method, a probe containing base-discriminating fluorescent (BDF) bases is added to a sample solution. BDF base-containing DNA usually shows only a weak fluorescence, but emits a strong blue fluorescence when it recognizes a target base at a specific site in a hybridized strand. By utilizing this feature, a simple mix-and-read SNP typing assay was achieved without any tedious probe-designing or washing processes for exclusion of hybridization error or any addition of DNA-modifying enzymes. This is very different from conventional methods. We simultaneously analyzed a number of samples with ease, with a high accuracy, using our BDF assay.

Graphical abstract: Simple SNP typing assay using a base-discriminating fluorescent probe

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Article information


Submitted
10 Nov 2005
Accepted
14 Dec 2005
First published
05 Jan 2006

Mol. BioSyst., 2006,2, 122-127
Article type
Method

Simple SNP typing assay using a base-discriminating fluorescent probe

A. Okamoto, K. Tainaka, Y. Ochi, K. Kanatani and I. Saito, Mol. BioSyst., 2006, 2, 122 DOI: 10.1039/B515923G

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