Jump to main content
Jump to site search
SCHEDULED MAINTENANCE Close the message box

Maintenance work is planned for Monday 16 August 2021 from 07:00 to 23:59 (BST).

Website performance may be temporarily affected and you may not be able to access some PDFs or images. If this does happen, refreshing your web browser should resolve the issue. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 9, 2006

On-line laser Raman spectroscopic probing of droplets engineered in microfluidic devices

Author affiliations

Abstract

Sub-nanolitre droplets engineered in microfluidic devices constitute ideal microreactors to investigate the kinetics of chemical reactions on the millisecond time scale. Up to date, fluorescence detection has been extensively used in chemistry and biology to probe reactants and resultant products within such nanodroplets. However, although fluorescence is a very sensitive technique, it lacks intrinsic specificity as frequently fluorescent labels need to be attached to the species of interest. This weakness can be overcome by using vibrational spectroscopy analysis. As an illustrative example, we use confocal Raman microspectroscopy in order to probe the concentration profiles of two interdiffusing solutes within nanolitre droplets transported through a straight microchannel. We establish the feasibility of the experimental method and discuss various aspects related to the space-time resolution and the quantitativeness of the Raman measurements. Finally, we demonstrate that the droplet internal molecular mixing is strongly affected by the droplet internal flow.

Graphical abstract: On-line laser Raman spectroscopic probing of droplets engineered in microfluidic devices

Article information


Submitted
22 Feb 2006
Accepted
22 May 2006
First published
06 Jun 2006

Lab Chip, 2006,6, 1140-1146
Article type
Paper

On-line laser Raman spectroscopic probing of droplets engineered in microfluidic devices

G. Cristobal, L. Arbouet, F. Sarrazin, D. Talaga, J. Bruneel, M. Joanicot and L. Servant, Lab Chip, 2006, 6, 1140 DOI: 10.1039/B602702D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements