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Issue 30, 2006
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Selection of DNA ligands for protein kinase C-δ

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Protein kinase Cs are a family of serine and threonine kinases that mediate a wide variety of cellular signalling processes such as cell growth, differentiation, apoptosis and tumor development. We have selected high-affinity DNA aptamers for PKCδ by capillary electrophoresis based SELEX (systematic evolution of ligands by exponential enrichment, CE-SELEX). We have demonstrated that fluorescently tagged PB9 aptamer can specifically recognize PKCδ under in vitro conditions. The Kd of the aptamer-protein binding is 122 nM. These aptamers will enable us to apply fluorescently labelled probes to study the spatiotemporal dynamics and activation of individual endogenous PKC isoforms during various cell signalling processes.

Graphical abstract: Selection of DNA ligands for protein kinase C-δ

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The article was received on 03 Apr 2006, accepted on 24 May 2006 and first published on 23 Jun 2006

Article type: Communication
DOI: 10.1039/B604778E
Chem. Commun., 2006, 3229-3231

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    Selection of DNA ligands for protein kinase C-δ

    P. Mallikaratchy, R. V. Stahelin, Z. Cao, W. Cho and W. Tan, Chem. Commun., 2006, 3229
    DOI: 10.1039/B604778E

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