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Issue 4, 2006
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Towards biochemical reaction monitoring using FT-IR synchrotron radiation

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Abstract

A lab-on-a-chip device made of CaF2 windows and SU-8 polymer was used for fluid lamination to achieve rapid mixing of two streamlines with a cross section of 300 × 5 µm each. Time resolved measurements of the induced chemical reaction was achieved by applying constant feeding low flow rates and by on-chip measurement at defined distances after the mixing point. Synchrotron IR microscopic detection was employed for direct and label-free monitoring of (bio)chemical reactions. Furthermore, using synchrotron IR microscopy the measurement spot could be reduced to the diffraction limit, thus maximizing time resolution in the experimental set-up under study. Based on computational fluid dynamic simulations the principle of the set-up is discussed. Experimental results on the basic hydrolysis of methyl chloroacetate proved the working principle of the experimental set-up. First results on the interaction between the antibiotic vancomycin and a tripeptide (Ac2KAA) involved in the build up of the membrane proteins of gram-positive bacteria are presented.

Graphical abstract: Towards biochemical reaction monitoring using FT-IR synchrotron radiation

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Publication details

The article was received on 05 Oct 2005, accepted on 06 Jan 2006 and first published on 27 Jan 2006


Article type: Paper
DOI: 10.1039/B514102H
Analyst, 2006,131, 489-494

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    Towards biochemical reaction monitoring using FT-IR synchrotron radiation

    N. Kaun, S. Kulka, J. Frank, U. Schade, M. J. Vellekoop, M. Harasek and B. Lendl, Analyst, 2006, 131, 489
    DOI: 10.1039/B514102H

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