Issue 11, 2005
From the journal:

Lab on a Chip

Microfluidic device as a new platform for immunofluorescent detection of viruses

Wen-Tso Liu,*a   Liang Zhu,a   Qi-Wei Qin,b   Qing Zhang,a   Hanhua Fengc  and  Simon Angd  

* Corresponding authors

a Division of Environmental Science and Engineering, National University of Singapore, Blk E1A, #07-03, Engineering Drive 2, Singapore
E-mail: cveliuwt@nus.edu.sg
Fax: +65-67791635
Tel: +65-68741315

b The State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-Sun University, Guangzhou, P. R. China

c Institute of Microelectronics, Singapore

d Department of Electrical Engineering and Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, USA

Abstract

A bead-based microfluidic device was developed and demonstrated to achieve rapid and sensitive enzyme-linked immunosorbent assay (ELISA) with quantum dots as the labeling fluorophore for virus detection. In comparison to standard ELISA performed on the same virus, the minimal detectable concentration of the target virus was improved from 360 to 22 ng mL−1, the detection time was shortened from >3.25 h to <30 min, and the amount of antibody consumed was reduced by a factor of 14.3.

Graphical abstract: Microfluidic device as a new platform for immunofluorescent detection of viruses

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Article information

DOI
https://doi.org/10.1039/B509086E
Article type
Technical Note
Submitted
27 Jun 2005
Accepted
27 Sep 2005
First published
04 Oct 2005

Lab Chip, 2005,5, 1327-1330

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Microfluidic device as a new platform for immunofluorescent detection of viruses

W. Liu, L. Zhu, Q. Qin, Q. Zhang, H. Feng and S. Ang, Lab Chip, 2005, 5, 1327 DOI: 10.1039/B509086E

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