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Issue 10, 2005
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An integrated microfluidic processor for single nucleotide polymorphism-based DNA computing

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Abstract

An integrated microfluidic processor is developed that performs molecular computations using single nucleotide polymorphisms (SNPs) as binary bits. A complete population of fluorescein-labeled DNA “answers” is synthesized containing three distinct polymorphic bases; the identity of each base (A or T) is used to encode the value of a binary bit (TRUE or FALSE). Computation and readout occur by hybridization to complementary capture DNA oligonucleotides bound to magnetic beads in the microfluidic device. Beads are loaded into sixteen capture chambers in the processor and suspended in place by an external magnetic field. Integrated microfluidic valves and pumps circulate the input DNA population through the bead suspensions. In this example, a program consisting of a series of capture/rinse/release steps is executed and the DNA molecules remaining at the end of the computation provide the solution to a three-variable, four-clause Boolean satisfiability problem. The improved capture kinetics, transfer efficiency, and single-base specificity enabled by microfluidics make our processor well-suited for performing larger-scale DNA computations.

Graphical abstract: An integrated microfluidic processor for single nucleotide polymorphism-based DNA computing

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Article information


Submitted
26 Apr 2005
Accepted
21 Jul 2005
First published
11 Aug 2005

Lab Chip, 2005,5, 1033-1040
Article type
Paper

An integrated microfluidic processor for single nucleotide polymorphism-based DNA computing

W. H. Grover and R. A. Mathies, Lab Chip, 2005, 5, 1033
DOI: 10.1039/B505840F

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