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Issue 4, 2005
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Laser induced disruption of bacterial spores on a microchip

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We report on the development of a laser based spore disruption method. Bacillus globigii spores were mixed with a laser light absorbing matrix and co-crystallized into 200-µm-wide and 20-µm-deep nanovials formed in a polydimethylsiloxane (PDMS) target plate. Surface tension effects were exploited to effect up to 125-fold spore enrichment. When the target zones were illuminated at atmospheric pressure with pulsed UV-laser light at fluences below 20 mJ cm−2 a change in spore morphology was observed within seconds. Post illumination PCR analysis suggests the release of endogenous DNA indicative of spore disruption. For laser fluences above 20 mJ cm−2, desorption of spores and fragments was also observed even without a matrix being employed. Desorbed material was collected in a PDMS flowcell attached to the target plate during laser illumination. This opens up a route towards the direct extraction of released DNA in an integrated spore disruption–PCR amplification microchip device.

Graphical abstract: Laser induced disruption of bacterial spores on a microchip

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Article information

13 Dec 2004
07 Feb 2005
First published
23 Feb 2005

Lab Chip, 2005,5, 374-377
Article type

Laser induced disruption of bacterial spores on a microchip

O. Hofmann, K. Murray, A. Wilkinson, T. Cox and A. Manz, Lab Chip, 2005, 5, 374
DOI: 10.1039/B418663J

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