Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 4, 2005
Previous Article Next Article

Laser induced disruption of bacterial spores on a microchip

Author affiliations

Abstract

We report on the development of a laser based spore disruption method. Bacillus globigii spores were mixed with a laser light absorbing matrix and co-crystallized into 200-µm-wide and 20-µm-deep nanovials formed in a polydimethylsiloxane (PDMS) target plate. Surface tension effects were exploited to effect up to 125-fold spore enrichment. When the target zones were illuminated at atmospheric pressure with pulsed UV-laser light at fluences below 20 mJ cm−2 a change in spore morphology was observed within seconds. Post illumination PCR analysis suggests the release of endogenous DNA indicative of spore disruption. For laser fluences above 20 mJ cm−2, desorption of spores and fragments was also observed even without a matrix being employed. Desorbed material was collected in a PDMS flowcell attached to the target plate during laser illumination. This opens up a route towards the direct extraction of released DNA in an integrated spore disruption–PCR amplification microchip device.

Graphical abstract: Laser induced disruption of bacterial spores on a microchip

Back to tab navigation

Article information


Submitted
13 Dec 2004
Accepted
07 Feb 2005
First published
23 Feb 2005

Lab Chip, 2005,5, 374-377
Article type
Communication

Laser induced disruption of bacterial spores on a microchip

O. Hofmann, K. Murray, A. Wilkinson, T. Cox and A. Manz, Lab Chip, 2005, 5, 374
DOI: 10.1039/B418663J

Social activity

Search articles by author

Spotlight

Advertisements