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Issue 4, 2005

Stacking due to ionic transport number mismatch during sample sweeping on microchips

Author affiliations

Abstract

Sample stacking can occur in isoconductive buffer systems as a result of ion transport mismatches that cause changes in buffer conductivity during electrophoresis. Fluorescence imaging was used to examine this effect in the sweeping of hydrophobic dyes with sodium dodecyl sulfate (SDS) on microchips. Imaging revealed the occurrence of a stacking effect in a sodium borate buffer system in which the sample buffer and SDS-containing run buffer had the same initial conductivity. Injected sample plugs were first swept by SDS micelles and the swept band was then stacked at the trailing end of the sample zone. This effect is due to changes in conductivity at both the front and back interfaces of the injected sample plug and can be modeled by moving boundary equations. Maximum signal enhancements of 86-, 160- and 560-fold were obtained for Rhodamine 560, Rhodamine B and Rhodamine 6G, respectively, by the combination of sweeping and stacking within a 1 cm section of microchannel. Based on sample sweeping/stacking and manipulation of the electric field polarity, a method of trapping and concentrating analyte from multiple injections was also demonstrated.

Graphical abstract: Stacking due to ionic transport number mismatch during sample sweeping on microchips

Article information


Submitted
25 Oct 2004
Accepted
31 Jan 2005
First published
07 Mar 2005

Lab Chip, 2005,5, 457-465
Article type
Paper

Stacking due to ionic transport number mismatch during sample sweeping on microchips

Y. Liu, R. S. Foote, S. C. Jacobson and J. M. Ramsey, Lab Chip, 2005, 5, 457 DOI: 10.1039/B416414H

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