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Issue 4, 2005
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Parallel nanoliter detection of cancer markers using polymer microchips

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Abstract

A general multipurpose microchip technology platform for point-of-care diagnostics has been developed. Real-time nucleic acid sequence-based amplification (NASBA) for detection of artificial human papilloma virus (HPV) 16 sequences and SiHa cell line samples was successfully performed in cyclic olefin copolymer (COC) microchips, incorporating supply channels and parallel reaction channels. Samples were distributed into 10 parallel reaction channels, and signals were simultaneously detected in 80 nl volumes. With a custom-made optical detection unit, the system reached a sensitivity limit of 10−6 µM for artificial HPV 16 sequences, and 20 cells µl−1 for the SiHa cell line. This is comparable to the detection limit of conventional readers, and clinical testing of biological samples in polymer microchips using NASBA is therefore possible.

Graphical abstract: Parallel nanoliter detection of cancer markers using polymer microchips

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Article information


Submitted
08 Oct 2004
Accepted
10 Jan 2005
First published
28 Jan 2005

Lab Chip, 2005,5, 416-420
Article type
Paper

Parallel nanoliter detection of cancer markers using polymer microchips

A. Gulliksen, L. Anders Solli, K. Stefan Drese, O. Sörensen, F. Karlsen, H. Rogne, E. Hovig and R. Sirevåg, Lab Chip, 2005, 5, 416
DOI: 10.1039/B415525D

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