Jump to main content
Jump to site search

Issue 4, 2005
Previous Article Next Article

Efficient solid-state NMR methods for measuring heteronuclear dipolar couplings in unoriented lipid membrane systems

Author affiliations

Abstract

Recently (Dvinskikh et al., J. Magn. Reson., 2003, 164, 165 and Dvinskikh et al., J. Magn. Reson., 2004, 168, 194), some of us introduced two efficient solid-state NMR techniques for the determination of heteronuclear dipolar couplings under magic-angle spinning (MAS). These two-dimensional (2D) recoupling methods have been applied previously to simple amino acids, and to columnar systems with high positional and orientational order. In this work, we show that the 2D MAS sequences produce unparalleled 1H–13C dipolar resolution in unoriented lipid membranes. The recoupling experiments were applied to hydrated dimyristoylphosphatidylcholine (DMPC) in the liquid-crystalline Lα phase, and the results agreed well with previous NMR investigations using specifically deuterated phospholipids.

Graphical abstract: Efficient solid-state NMR methods for measuring heteronuclear dipolar couplings in unoriented lipid membrane systems

Back to tab navigation

Article information


Submitted
01 Dec 2004
Accepted
12 Jan 2005
First published
24 Jan 2005

Phys. Chem. Chem. Phys., 2005,7, 607-613
Article type
Paper

Efficient solid-state NMR methods for measuring heteronuclear dipolar couplings in unoriented lipid membrane systems

S. V. Dvinskikh, V. Castro and D. Sandström, Phys. Chem. Chem. Phys., 2005, 7, 607
DOI: 10.1039/B418131J

Social activity

Search articles by author

Spotlight

Advertisements