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Issue 10, 2005
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Pumping of mammalian cells with a nozzle-diffuser micropump

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Abstract

We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.

Graphical abstract: Pumping of mammalian cells with a nozzle-diffuser micropump

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Supplementary files

Article information


Submitted
31 Mar 2005
Accepted
04 Aug 2005
First published
23 Aug 2005

Lab Chip, 2005,005, 1083-1088
Article type
Paper

Pumping of mammalian cells with a nozzle-diffuser micropump

C. Yamahata, C. Vandevyver, F. Lacharme, P. Izewska, H. Vogel, R. Freitag and M. A. M. Gijs, Lab Chip, 2005, 005, 1083
DOI: 10.1039/B504468E

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