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Issue 27-28, 2005
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Indicator displacement assays that detect bilayer membranes enriched in phosphatidylserine

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Abstract

Three indicator displacement assays are described for the detection of phosphatidylserine in a bilayer membrane. A series of Zn2+-dipicolylamine coordination compounds are used to bind selectively to the phosphatidylserine and act as a colorimetric chemosensing ensemble when combined with the UV-Vis indictor pyrocatechol violet. A similar displacement assay uses a coumarin methylsulfonate derivative as a fluorescent indicator, and a third assay involves quenching of calcein fluorescence by Cu2+ and subsequent fluorescence restoration upon addition of phosphatidylserine. In the best case, vesicle membranes containing as little as 5% phosphatidylserine could be detected under physiologically relevant conditions using as little as 10 µM sensing ensemble, and two of the three systems allow vesicles containing 50% phosphatidylserine to be detected by the naked eye.

Graphical abstract: Indicator displacement assays that detect bilayer membranes enriched in phosphatidylserine

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Publication details

The article was received on 12 Feb 2005, accepted on 18 Mar 2005 and first published on 05 Apr 2005


Article type: Paper
DOI: 10.1039/B500522A
J. Mater. Chem., 2005,15, 2707-2713

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    Indicator displacement assays that detect bilayer membranes enriched in phosphatidylserine

    R. G. Hanshaw, E. J. O'Neil, M. Foley, R. T. Carpenter and B. D. Smith, J. Mater. Chem., 2005, 15, 2707
    DOI: 10.1039/B500522A

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