Jump to main content
Jump to site search

Issue 2, 2005
Previous Article Next Article

Arsenic compounds in the haemolymph of the Dungeness crab, Cancer magister, as determined by using HPLC on-line with inductively coupled plasma mass spectrometry

Author affiliations

Abstract

Arsenobetaine, two arsenosugars, dimethylarsinate and several unidentified arsenic species were detected in extracts of the haemolymph of the Dungeness crab, Cancer magister, by using HPLC-ICP-MS. This is the first report of the presence of arsenosugars in the haemolymph/blood of marine animals. Total, extractable and residual arsenic concentrations were determined by ICP-MS. The concentration of total arsenic was in the range of 1.4–3.8 μg ml−1. Nearly all (98%) the arsenic was found to be extractable, and accounted for primarily by arsenobetaine, two arsenosugars and dimethylarsinate. The results demonstrate that arsenic compounds present in the diet of crabs are not fully metabolized in the gut. They are, at least partly, taken up into the haemolymph. The concurrence of arsenobetaine and arsenosugars suggests that the use of repeated haemolymph sampling in crustaceans could facilitate investigations into the kinetics of the biotransformation pathways of arsenic compounds. Finally, the present study clearly demonstrates the unique capabilities of HPLC-ICP-MS for the detection and identification of minor arsenic components amongst the predominant arsenobetaine.

Graphical abstract: Arsenic compounds in the haemolymph of the Dungeness crab, Cancer magister, as determined by using HPLC on-line with inductively coupled plasma mass spectrometry

Back to tab navigation

Publication details

The article was received on 10 Aug 2004, accepted on 15 Dec 2004 and first published on 17 Jan 2005


Article type: Paper
DOI: 10.1039/B412311E
J. Environ. Monit., 2005,7, 122-126

  •   Request permissions

    Arsenic compounds in the haemolymph of the Dungeness crab, Cancer magister, as determined by using HPLC on-line with inductively coupled plasma mass spectrometry

    U. Nørum, V. W.-M. Lai, S. A. Pergantis and W. R. Cullen, J. Environ. Monit., 2005, 7, 122
    DOI: 10.1039/B412311E

Search articles by author

Spotlight

Advertisements