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Issue 7, 2005
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Charge-state resolved mid-infrared spectroscopy of a gas-phase protein

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Abstract

Infrared spectra of a 104 amino-acid protein in the gas phase as a function of its charge state are presented. The spectra contain clearly resolvable bands in the amide I and II spectral regions, as well as a band at 1483 cm−1, which is not observed in solution phase spectroscopy and is especially prominent for the higher charge states. Compared to solution, the amide I band is blue-shifted and the amide II band red-shifted, as expected for species in an environment with reduced hydrogen bonding. The band positions are suggestive of a mostly α-helical structure of the protein and their widths are comparable to those in solution, suggesting a similar conformational distribution.

Graphical abstract: Charge-state resolved mid-infrared spectroscopy of a gas-phase protein

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Article information


Submitted
15 Feb 2005
Accepted
03 Mar 2005
First published
07 Mar 2005

Phys. Chem. Chem. Phys., 2005,7, 1345-1348
Article type
Communication

Charge-state resolved mid-infrared spectroscopy of a gas-phase protein

J. Oomens, N. Polfer, D. T. Moore, L. van der Meer, A. G. Marshall, J. R. Eyler, G. Meijer and G. von Helden, Phys. Chem. Chem. Phys., 2005, 7, 1345
DOI: 10.1039/B502322J

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