Time-domain fluorescence lifetime imaging applied to biological tissue

Dan Elson; Jose Requejo-Isidro; Ian Munro; Fred Reavell; Jan Siegel; Klaus Suhling; Paul Tadrous; Richard Benninger; Peter Lanigan; James McGinty; Clifford Talbot; Bebhinn Treanor; Stephen Webb; Ann Sandison; Andrew Wallace; Dan Davis; John Lever; Mark Neil; David Phillips; Gordon Stamp; Paul French

doi:10.1039/B316456J

Time-domain fluorescence lifetime imaging applied to biological tissue

Dan Elson,^a Jose Requejo-Isidro,^a Ian Munro,^a Fred Reavell,^a Jan Siegel,^a Klaus Suhling,^a Paul Tadrous,^a Richard Benninger,^a Peter Lanigan,^a James McGinty,^a Clifford Talbot,^a Bebhinn Treanor,^a Stephen Webb,^a Ann Sandison,^a Andrew Wallace,^a Dan Davis,^a John Lever,^a Mark Neil,^a David Phillips,^a Gordon Stamp^a and Paul French^a

Author affiliations

^a Photonics Group, Physics Department, Imperial College London, London, UK
E-mail: ds.elson@imperial.ac.uk

Abstract

Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide information, not only concerning the localisation of specific fluorophores, but also about the local fluorophore environment. It may be implemented in scanning confocal or multi-photon microscopes, or in wide-field microscopes and endoscopes. When applied to tissue autofluorescence, it reveals intrinsic excellent contrast between different types and states of tissue. This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue.

This article is part of the themed collection: 10th Congress of the European Society for Photobiology

Photochemical & Photobiological Sciences

Time-domain fluorescence lifetime imaging applied to biological tissue

Abstract

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Time-domain fluorescence lifetime imaging applied to biological tissue

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