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Issue 2, 2004
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Photobiological modulation of cell attachment via cytochrome c oxidase

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Abstract

The number of cells attached to glass substrates increases if HeLa cell suspensions are irradiated with monochromatic visible-to-near infrared radiation (600–860 nm, 52 J m−2) prior to plating. The well-structured relationship between this biological response and the radiation wavelength (action spectrum with maxima at 620, 680, 760, and 820 nm) suggests the existence of a photoacceptor responsible for the enhancement of attachment (presumably cytochrome c oxidase, the terminal enzyme of the respiratory chain) and, secondly, the existence of signaling pathways between the mitochondria, the plasma membrane, and the nucleus of the cell. Treating the cell suspension with ouabain (a Na+, K+-ATPase inhibitor), amiloride (an inhibitor of N+/H+ exchangers), or sodium azide (a cytochrome c oxidase inhibitor) prior to irradiation significantly modifies the action spectrum of cell attachment enhancement. The action of the chemicals under study also depends on their concentration and radiation fluence. Our results point to the existence of at least three signaling pathways (reaction channels) relating together the cell attachment, the respiratory chain, and the Na+, K+-ATPase and N+/H+ exchanger activities.

Graphical abstract: Photobiological modulation of cell attachment via cytochrome c oxidase

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Publication details

The article was received on 30 May 2003, accepted on 22 Oct 2003 and first published on 12 Nov 2003


Article type: Paper
DOI: 10.1039/B306126D
Citation: Photochem. Photobiol. Sci., 2004,3, 211-216
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    Photobiological modulation of cell attachment via cytochrome c oxidase

    T. I. Karu, L. V. Pyatibrat and G. S. Kalendo, Photochem. Photobiol. Sci., 2004, 3, 211
    DOI: 10.1039/B306126D

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