Issue 20, 2004
From the journal:

Chemical Communications

Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis†

Russell J. Cox,*a   Frank Glod,a   Deirdre Hurley,a   Colin M. Lazarus,b   Thomas. P. Nicholson,a   Brian A. M. Rudd,‡c   Thomas J. Simpson,a   Barrie Wilkinson‡c  and  Ying Zhanga  

* Corresponding authors

a School of Chemistry, University of Bristol, Cantock's Close, Bristol, UK
E-mail: r.j.cox@bris.ac.uk
Fax: +44 (0)117 9298611

b School of Biological Sciences, University of Bristol, Woodland Road, Bristol

c GlaxoSmithKline, Gunnels Wood Road, Stevenage, Herts

Abstract

PCR primers designed to selectively amplify the unique C-methyltransferase domain of fungal polyketide synthases were used to selectively clone a polyketide synthase gene involved in the biosynthesis of the squalene synthase inhibitor squalestatin S1 1, heterologous expression of which led to the biosynthesis of the squalestatin side-chain.

Graphical abstract: Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis

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Article information

DOI
https://doi.org/10.1039/B411973H
Article type
Communication
Submitted
04 Aug 2004
Accepted
03 Sep 2004
First published
24 Sep 2004

Chem. Commun., 2004, 2260-2261

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Rapid cloning and expression of a fungal polyketide synthase gene involved in squalestatin biosynthesis

R. J. Cox, F. Glod, D. Hurley, C. M. Lazarus, Thomas. P. Nicholson, B. A. M. Rudd, T. J. Simpson, B. Wilkinson and Y. Zhang, Chem. Commun., 2004, 2260 DOI: 10.1039/B411973H

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