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Issue 6, 2004
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Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems

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Abstract

A one-step immunomagnetic separation technique was performed on a microfluidic platform for the isolation of specific cells from blood samples. The cell isolation and purification studies targeted T cells, as a model for low abundance cells (about 1∶10,000 cells), with more dilute cells as the ultimate goal. T cells were successfully separated on-chip from human blood and from reconstituted blood samples. Quantitative polymerase chain reaction analysis of the captured cells was used to characterize the efficiency of T cell capture in a variety of flow path designs. Employing many (4–8), 50 µm deep narrow channels, with the same overall cross section as a single, 3 mm wide channel, was much more effective in structuring dense enough magnetic bead beds to trap cells in a flowing stream. The use of 8-multiple bifurcated flow paths increased capture efficiencies from ∼20 up to 37%, when compared to a straight 8-way split design, indicating the value of ensuring uniform flow distribution into each channel in a flow manifold for effective cell capture. Sample flow rates of up to 3 µL min−1 were evaluated in these capture beds.

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Publication details

The article was received on 21 Jun 2004, accepted on 24 Aug 2004 and first published on 11 Nov 2004


Article type: Paper
DOI: 10.1039/B409366F
Lab Chip, 2004,4, 614-618

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    Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems

    V. I. Furdui and D. J. Harrison, Lab Chip, 2004, 4, 614
    DOI: 10.1039/B409366F

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