Issue 5, 2004

“Microcanals” for micropipette access to single cells in microfluidic environments

Abstract

We demonstrate the fabrication and operation of “microcanals” (i.e. open-air microfluidic channels without a roof), which enable micropipette manipulation and probing of cells within a microfluidic environment. The microcanal devices are fabricated in PDMS on glass substrates using a PDMS membrane transferring technique. Here we show patch-clamp electrophysiological recording and intracellular dye injection performed on cells seeded in microcanals.

Article information

Article type
Paper
Submitted
01 Apr 2004
Accepted
26 Apr 2004
First published
23 Jul 2004

Lab Chip, 2004,4, 420-424

“Microcanals” for micropipette access to single cells in microfluidic environments

C. Hsu, C. Chen and A. Folch, Lab Chip, 2004, 4, 420 DOI: 10.1039/B404956J

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