Analysis of nickel species in cytosols of normal and malignant human colonic tissues using two dimensional liquid chromatography with ICP-sector field MS detection

Abstract

A method has been developed that allows the nickel species in healthy and neoplastic tissues from cancer subjects to be compared. It is based on the coupling of sequentially applied anion-exchange and size-exclusion chromatography using an ICP-sector field mass spectrometer as detector. The method allows the resolution of seven nickel species in tissue extracts, which can be considered as fingerprints of nickel speciation. A qualitative difference in Ni-binding biomolecules between cytosols of normal and malignant colon tissues could not be found.