Fabrication of 2D arrays of giant liposomes on solid substrates by microcontact printing

Abstract

We have developed a novel technique for preparation of 2D arrays of giant liposomes on solid substrates by using a combination of micro-patterning of ITO glass slides with lipid solution and electroswelling. Microcontact printing with silicone elastomer stamps is used with lipid solutions “inks” to produce lipid patterns on electro-conductive solid substrates. Special attention is paid to the wetting properties of the ink with respect to the stamp and the solid substrates. The average diameter of the giant liposomes produced by electroswelling of the lipid pattern is determined by size of the micro-pattern features. The technique can be used for preparation of giant liposomes of narrow size distribution.