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Issue 8, 2003
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Understanding protease catalysed solid phase peptide synthesis

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A protease (thermolysin) was used to directly synthesise a number of dipeptides from soluble Fmoc-amino acids onto a solid support (PEGA1900) in bulk aqueous media, often in very good yields. This shift in equilibrium toward synthesis is remarkable because for soluble dipeptides in aqueous solution hydrolysis rather than synthesis is observed. Three possible reasons for the equilibrium shift were considered: (i) using a solid support makes it easy to use an excess of reagents, so mass action contributes towards synthesis; (ii) reduction in the unfavourable hydrophobic hydration of the Fmoc group within the solid support compared with the free amino acid in solution and (iii) suppression of the ionization of amino groups linked to the solid phase due to mutual electrostatic repulsion. It was found that under the conditions studied the second effect was most important.

Graphical abstract: Understanding protease catalysed solid phase peptide synthesis

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Publication details

The article was received on 05 Dec 2002, accepted on 04 Mar 2003 and first published on 19 Mar 2003

Article type: Paper
DOI: 10.1039/B211890D
Org. Biomol. Chem., 2003,1, 1277-1281

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    Understanding protease catalysed solid phase peptide synthesis

    R. V. Ulijn, N. Bisek, P. J. Halling and S. L. Flitsch, Org. Biomol. Chem., 2003, 1, 1277
    DOI: 10.1039/B211890D

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