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Issue 4, 2003
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GlycoChip: multiarray for the study of carbohydrate-binding proteins

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Biotinylated glycoconjugates which were designed as oligosaccharides attached to 30 kDa polyacrylamide were coated on a microarray platform XNAonGOLD™, which was developed earlier for an oligonucleotide assay. The specificity of antibodies to carbohydrate antigens was analyzed using the glyco-microarray. Comparison of the obtained results with those of common 96-well plate ELISA completely coincided with the found antibody specificities. However, parameters such as the analytical sensitivity of the method and the amount of biotinylated material coated on the microarray platform proved to be worse than expected.

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Publication details

The article was received on 27 May 2003, accepted on 01 Aug 2003 and first published on 18 Aug 2003

Article type: Paper
DOI: 10.1039/B305963D
Lab Chip, 2003,3, 260-265

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    GlycoChip: multiarray for the study of carbohydrate-binding proteins

    O. E. Galanina, M. Mecklenburg, N. E. Nifantiev, G. V. Pazynina and N. V. Bovin, Lab Chip, 2003, 3, 260
    DOI: 10.1039/B305963D

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