Issue 17, 2002
From the journal:

Chemical Communications

Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelation†

Imogen Horsey,a   Yamuna Krishnan-Ghosha  and  Shankar Balasubramanian*a  

* Corresponding authors

a University Chemical Laboratories, University of Cambridge, Lensfield Road, Cambridge, UK
E-mail: sb10031@cam.ac.uk
Fax: 44 1223 336913
Tel: 44 1223 336347

Abstract

Metal chelating iminodiacetic acid moieties were appended to abutting ends of two 9-mer oligonucleotides designed to hybridize contiguously on an 18-mer target. Chelation of 1 equivalent of Gd3+ cooperatively increases affinities of the oligonucleotides to the single stranded DNA target as evidenced by a 15 °C increase in Tm of the complex.

Graphical abstract: Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelation

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/B206054J
Article type
Communication
Submitted
26 Jun 2002
Accepted
19 Jul 2002
First published
05 Aug 2002

Chem. Commun., 2002, 1950-1951

Permissions

Request permissions

Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelation

I. Horsey, Y. Krishnan-Ghosh and S. Balasubramanian, Chem. Commun., 2002, 1950 DOI: 10.1039/B206054J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements