Issue 10, 2002
From the journal:

Chemical Communications

Artificial regulation of transcription applying carbohydrate–lectin interaction

Kazunori Matsuura,ab   Katsuhiro Hayashia  and  Kazukiyo Kobayashi*a  

* Corresponding authors

a Department of Molecular Design, Graduate School of Engineering, Nagoya University, Chikusa-ku, Nagoya 464-8603, Japan
E-mail: kobayash@mol.nagoya-u.ac.jp

b Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan

Abstract

A complex of plasmid DNA–lactose conjugate with lectin is proposed as an artificial system to control transcription activity: the in vitro transcription of DNA in the conjugate with T7 RNA polymerase was repressed in the presence of RCA120, and then the transcription ability was recovered by adding lactose or a lactose-carrying polymer to the repression system.

Graphical abstract: Artificial regulation of transcription applying carbohydrate–lectin interaction

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Article information

DOI
https://doi.org/10.1039/B202579P
Article type
Communication
Submitted
13 Mar 2002
Accepted
12 Apr 2002
First published
23 Apr 2002

Chem. Commun., 2002, 1140-1141

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Artificial regulation of transcription applying carbohydrate–lectin interaction

K. Matsuura, K. Hayashi and K. Kobayashi, Chem. Commun., 2002, 1140 DOI: 10.1039/B202579P

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