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Issue 1, 2002
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An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities

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Abstract

This paper presents the development and characterization of an integrated microfluidic biochemical detection system for fast and low-volume immunoassays using magnetic beads, which are used as both immobilization surfaces and bio-molecule carriers. Microfluidic components have been developed and integrated to construct a microfluidic biochemical detection system. Magnetic bead-based immunoassay, as a typical example of biochemical detection and analysis, has been successfully performed on the integrated microfluidic biochemical analysis system that includes a surface-mounted biofilter and electrochemical sensor on a glass microfluidic motherboard. Total time required for an immunoassay was less than 20 min including sample incubation time, and sample volume wasted was less than 50 μl during five repeated assays. Fast and low-volume biochemical analysis has been successfully achieved with the developed biofilter and immunosensor, which is integrated to the microfluidic system. Such a magnetic bead-based biochemical detection system, described in this paper, can be applied to protein analysis systems.

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Article information


Submitted
20 Aug 2001
Accepted
02 Nov 2001
First published
06 Dec 2001

Lab Chip, 2002,2, 27-30
Article type
Paper

An integrated microfluidic biochemical detection system for protein analysis with magnetic bead-based sampling capabilities

J. Choi, K. W. Oh, J. H. Thomas, W. R. Heineman, H. B. Halsall, J. H. Nevin, A. J. Helmicki, H. T. Henderson and C. H. Ahn, Lab Chip, 2002, 2, 27
DOI: 10.1039/B107540N

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