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Issue 17, 2002
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Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelation

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Abstract

Metal chelating iminodiacetic acid moieties were appended to abutting ends of two 9-mer oligonucleotides designed to hybridize contiguously on an 18-mer target. Chelation of 1 equivalent of Gd3+ cooperatively increases affinities of the oligonucleotides to the single stranded DNA target as evidenced by a 15 °C increase in Tm of the complex.

Graphical abstract: Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelation

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Publication details

The article was received on 26 Jun 2002, accepted on 19 Jul 2002 and first published on 05 Aug 2002


Article type: Communication
DOI: 10.1039/B206054J
Citation: Chem. Commun., 2002,0, 1950-1951

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    Enhanced cooperative binding of oligonucleotides to form DNA duplexes mediated by metal ion chelation

    I. Horsey, Y. Krishnan-Ghosh and S. Balasubramanian, Chem. Commun., 2002, 0, 1950
    DOI: 10.1039/B206054J

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