A novel system for the preparation of orphan receptor ligand peptides

Osamu Nishimura; Nobuyuki Koyama; Takashi Itoh; Masato Suenaga; Takeo Moriya; Masanori Miwa; Yoko Tanaka; Chieko Kitada; Masahiko Fujino

doi:10.1039/B009566O

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A novel system for the preparation of orphan receptor ligandpeptides¹

Osamu Nishimura,*^a Nobuyuki Koyama,^b Takashi Itoh,^b Masato Suenaga,^b Takeo Moriya,^b Masanori Miwa,^b Yoko Tanaka,^b Chieko Kitada^a and Masahiko Fujino^a

Author affiliations

* Corresponding authors

^a Discovery Research Laboratories, Pharmaceutical Discovery Research Division, Takeda Chemical Industries, Ltd., Wadai-10, Tsukuba, Ibaraki, Japan

^b Discovery Research Laboratories, Pharmaceutical Discovery Research Division, Takeda Chemical Industries, Ltd., Yodogawa-ku, Osaka, Japan

Abstract

A new system is established for the preparation of biologically active recombinant peptides. The system consists of two newly developed chemical methods in combination with recombinant DNA technology. One method is a cleavage reaction by cyanylation from a recombinant fusion protein. The other is a scission reaction of an N-terminal extra-methionine by transamination from a recombinant peptide. This system is successfully established for the synthesis of three orphan receptor ligand peptides; apelin, prolactin-releasing peptide, and galanin-like peptide, isolated in our Research Division. The results show that this system is very versatile and might be useful in the preparation of biologically active peptides.

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Article information

DOI: https://doi.org/10.1039/B009566O
Article type: Paper
Submitted: 28 Nov 2000
Accepted: 26 Jun 2001
First published: 02 Aug 2001

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J. Chem. Soc., Perkin Trans. 1, 2001, 1960-1968

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A novel system for the preparation of orphan receptor ligand peptides

O. Nishimura, N. Koyama, T. Itoh, M. Suenaga, T. Moriya, M. Miwa, Y. Tanaka, C. Kitada and M. Fujino, J. Chem. Soc., Perkin Trans. 1, 2001, 1960 DOI: 10.1039/B009566O

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Journal of the Chemical Society, Perkin Transactions 1