Issue 24, 2001
From the journal:

Chemical Communications

Expansion of structural and functional diversities of DNA using new 5-substituted deoxyuridine derivatives by PCR with superthermophilic KOD Dash DNA polymerase†

Hiroaki Sawai,*a   Akiko N. Ozaki,a   Fumie Satoh,a   Tutomu Ohbayashi,a   Mohammad M. Masuda  and  Hiroaki Ozakia  

* Corresponding authors

a Department of Applied Chemistry, Gunma University, Kiryu, Gunma, Japan
E-mail: sawai@chem.gunma-u.ac.jp

Abstract

KOD Dash DNA polymerase can accept triphosphates of new deoxyuridine derivatives bearing a C5-substituent group via an α-methylene linker as a substrate in the polymerase chain reaction (PCR) yielding the corresponding functionalized DNA effectively, while other conventional DNA polymerases cannot tolerate the modification of the substrate.

Graphical abstract: Expansion of structural and functional diversities of DNA using new 5-substituted deoxyuridine derivatives by PCR with superthermophilic KOD Dash DNA polymerase

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/B107838K
Article type
Communication
Submitted
30 Aug 2001
Accepted
26 Oct 2001
First published
14 Nov 2001

Chem. Commun., 2001, 2604-2605

Permissions

Request permissions

Expansion of structural and functional diversities of DNA using new 5-substituted deoxyuridine derivatives by PCR with superthermophilic KOD Dash DNA polymerase

H. Sawai, A. N. Ozaki, F. Satoh, T. Ohbayashi, M. M. Masud and H. Ozaki, Chem. Commun., 2001, 2604 DOI: 10.1039/B107838K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements