Issue 19, 2001
From the journal:

Chemical Communications

Highly efficient oligosaccharide synthesis on water-soluble polymeric primers by recombinant glycosyltransferases immobilised on solid supports

Susumu Nishiguchi,a   Kuriko Yamada,a   Yoshitoshi Fuji,b   Shigeo Shibatani,a   Atushi Todaa  and  Shin-Ichiro Nishimura*b  

* Corresponding authors

a Sapporo Laboratory for Glycocluster Project, Japan Bioindustry Association, Sapporo, Japan

b Laboratory for Bio-Macromolecular Chemistry, Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan.
E-mail: shin@glyco.sci.hokudai.ac.jp

Abstract

Recombinant β-1,4-galactosyltransferase (β1,4-GalT) and α-2,6-sialyltransferase (α2,6-SiaT) immobilised covalently with activated Sepharose beads were employed for the practical synthesis of a trisaccharide derivative, Neu5Acα(2→6)Galβ(1→4)GlcNAcβ-O-(CH2) 6-NH2, on a water-soluble primer having GlcNAc residues through a α-chymotrypsin-sensitive linker.

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Article information

DOI
https://doi.org/10.1039/B104896C
Article type
Communication
Submitted
04 Jun 2001
Accepted
15 Aug 2001
First published
17 Sep 2001

Chem. Commun., 2001, 1944-1945

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Highly efficient oligosaccharide synthesis on water-soluble polymeric primers by recombinant glycosyltransferases immobilised on solid supports

S. Nishiguchi, K. Yamada, Y. Fuji, S. Shibatani, A. Toda and S. Nishimura, Chem. Commun., 2001, 1944 DOI: 10.1039/B104896C

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