Issue 7, 2001
From the journal:

Analyst

Investigation of microcontact transfer of proteins from a selectively plasma treated elastomer stamp by fluorescence microscopy and force microscopy

Xizeng Feng,a   Clive J. Roberts,*a   David A. Armitage,a   Martyn C. Davies,a   Saul J. B. Tendler,a   Stephanie Allena  and  Philip M. Williamsa  

* Corresponding authors

a Laboratory of Biophysics and Surface Analysis, Department of Pharmaceutical Sciences, The University of Nottingham, University Park, Nottingham, UK
E-mail: Clive.Roberts@nottingham.ac.uk

Abstract

Selective plasma treatment of the recessed regions of the elastomer stamps is shown to alter the resultant protein patterns. Fluorescence microscopy is demonstrated to be an excellent tool to discriminate between regions of microcontact printed fluorescent dye-labelled albumin in polystyrene. Atomic force microscopy and shear force microscopy are used to provide high-resolution images of the patterned protein layers. The formation and characteristics of the patterns formed by these alternative strategies is discussed.

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Article information

DOI
https://doi.org/10.1039/B102269P
Article type
Paper
Submitted
09 Mar 2001
Accepted
18 May 2001
First published
15 Jun 2001

Analyst, 2001,126, 1100-1104

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Investigation of microcontact transfer of proteins from a selectively plasma treated elastomer stamp by fluorescence microscopy and force microscopy

X. Feng, C. J. Roberts, D. A. Armitage, M. C. Davies, S. J. B. Tendler, S. Allen and P. M. Williams, Analyst, 2001, 126, 1100 DOI: 10.1039/B102269P

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