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Issue 8, 2001
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An integrated electrochemical capillary liquid chromatography–dual microelectrode system for bromine based reaction detection

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Abstract

Amperometric reaction detection of model peptides containing either a thiol or disulfide was successfully achieved with a novel coupled capillary liquid chromatography–dual microelectrode system. Detection is facilitated via dual platinum wire electrodes fabricated directly on a liquid chromatography capillary column. The detection strategy is based on the electrogeneration of bromine at the upstream working electrode followed by reduction of the bromine to bromide at the downstream working electrode. When bromine reacts with analyte eluting from the capillary column, the amount of bromine that reaches the downstream electrode decreases. As a result, the current at the downstream electrode will decrease in proportion to the amount of analyte eluting from the capillary column. Glutathione, glutathione disulfide, α-TGF and oxytocin were used to characterize the system and to determine system parameters. The feasibility of the determination of disulfide containing peptides is demonstrated with glutathione disulfide, α-TGF and oxytocin.

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Article information


Submitted
12 Feb 2001
Accepted
12 May 2001
First published
25 Jun 2001

Analyst, 2001,126, 1252-1256
Article type
Paper

An integrated electrochemical capillary liquid chromatography–dual microelectrode system for bromine based reaction detection

H. Zhou, L. A. Holland and P. Liu, Analyst, 2001, 126, 1252
DOI: 10.1039/B101480N

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