Jump to main content
Jump to site search

Issue 7, 2001
Previous Article Next Article

On-line continuous monitoring of glucose or lactate by ultraslow microdialysis combined with a flow-through nanoliter biosensor based on poly(m-phenylenediamine) ultra-thin polymer membrane as enzyme electrode

Author affiliations

Abstract

A miniaturised flow-through biosensor with a cell volume of only a few nanoliters was developed in our laboratory. The biosensor can be directly coupled to a microdialysis or ultrafiltration probe. Sampling and continuous on-line monitoring can thus be carried out at submicroliter levels and as a consequence quantitative recoveries of the analyte of interest are achieved. Via this method excessive calibration procedures, as are necessary with conventional microdialysis, are avoided. Here, the construction and the performance of such a biosensor for the continuous on-line monitoring of glucose and lactate will be presented. The biosensor is based on the amperometric detection of hydrogen peroxide after conversion of the analyte of interest by an immobilised oxidoreductase enzyme. Immobilisation of the enzyme is performed through electropolymerisation of m-phenylenediamine. Strategies to improve the performance (e.g. linearity, selectivity and stability) of the miniaturised biosensor are discussed and ex vivo and in vivo experiments carried out thus far demonstrate the potential of this miniaturised flow-through biosensor.

Back to tab navigation

Article information


Submitted
11 Jan 2001
Accepted
27 Apr 2001
First published
13 Jun 2001

Analyst, 2001,126, 1073-1079
Article type
Paper

On-line continuous monitoring of glucose or lactate by ultraslow microdialysis combined with a flow-through nanoliter biosensor based on poly(m-phenylenediamine) ultra-thin polymer membrane as enzyme electrode

M. M. Rhemrev-Boom, M. A. Jonker, K. Venema, G. Jobst, R. Tiessen and J. Korf, Analyst, 2001, 126, 1073
DOI: 10.1039/B100452M

Social activity

Search articles by author

Spotlight

Advertisements