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Issue 9, 2001
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This work describes the separation of the enantiomeric forms of Se-amino acids by reversed phase liquid chromatography following formation of the diastereomers using 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (Marfey's reagent) as the derivatizing agent. Optimization of the chromatographic conditions by UV absorption at 340 nm (λmax for Marfey's derivatives) was possible. The final operating conditions involved the use of 50% (v/v) MeOH at pH 5.3 (acetic acidsodium acetate), which provided a good separation of the diastereomers of Se-methionine within 26 min. Slight modification of the sample introduction system by on-line dilution (1∶4) of the chromatographic eluent using a PTFE tee piece was required for good performance of the ICP-MS. Final instrumental set-up involved the use of both detectors on-line (diode array and ICP-MS). Under these conditions, detection limits at the ppb level (11 ppb as Se in the Se-methionine diastereomer) using ICP-MS detection were obtained with negligible band broadening caused by the on-line dilution. The methodology was finally applied to the qualitative identification of D and L enantiomers in selenium nutritional supplements (selenized yeast and tablets) and a pharmaceutical preparation.

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Article information

17 Apr 2001
03 Jul 2001
First published
22 Aug 2001

J. Anal. At. Spectrom., 2001,16, 945-950
Article type

Resolution of seleno-amino acid optical isomers using chiral derivatization and inductively coupled plasma mass spectrometric (ICP-MS) detection

M. Montes-Bayón, C. B'Hymer, C. Ponce de León and J. A. Caruso, J. Anal. At. Spectrom., 2001, 16, 945
DOI: 10.1039/B103360N

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