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Issue 11, 2001
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Determination of the complex stability of zinc with carbonic anhydrase in sea-water

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Abstract

Carbonic anhydrase (CA) is inactive unless associated with zinc, with possible substitution by cobalt. In this work, the complexation of zinc by CA was determined in sea-water using cathodic stripping voltammetry (CSV) with ligand competition. The zinc was found to be released from the CA over a period of 3 h when equilibrated with a competing complexing ligand and the complex was re-formed with the CA when zinc was added. A value of 8.90 ± 0.27 was found for logKZnCA where KZnCA is the conditional stability constant for the complex of Zn2+ with CA in pH 8 sea-water. A value for the molecular weight of CA was calculated from its equivalent ligand concentration (in nM) obtained by titrations with zinc at various CA concentrations (1–4 mg l−1). The value found (34740 g mol−1) for the molecular weight is consistent with values found previously by other methods (29000–31000 g mol−1) confirming that the stoichiometry of the complex between zinc and CA is 1∶1. This work confirms that the zinc–CA complex is reversible and that the interaction between zinc and CA can be determined using CSV with ligand competition.

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Publication details

The article was received on 19 Jun 2001, accepted on 20 Aug 2001 and first published on 10 Oct 2001


Article type: Paper
DOI: 10.1039/B105364G
Analyst, 2001,126, 2036-2039

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    Determination of the complex stability of zinc with carbonic anhydrase in sea-water

    G. Sarthou, C. M. G. van den Berg and U. Riebesell, Analyst, 2001, 126, 2036
    DOI: 10.1039/B105364G

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