Issue 11, 2000
From the journal:

Analyst

A fluorescent-labeled

Yuya Ohuchi,a   Yoshiki Katayama*a  and  Mizuo Maedaa  

* Corresponding authors

a Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, Japan
E-mail: ykatatcm@mbox.nc.kyushu-u.ac.jp

Abstract

A fluorescent-labeled oligopeptide (DACM-CLRRASLK-fluorescein), containing a consensus amino acid sequence (RRXSL) of cyclic AMP (cAMP) dependent protein kinase A (PKA) substrate-proteins, was designed. The fluorescent peptide was a good substrate of PKA, and the phosphorylation of its serin residue caused an intensive change in fluorescent intensity. We expect that the peptide will be useful as a fluorescent indicator for monitoring PKA activity in living cells.

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Article information

DOI
https://doi.org/10.1039/B007152H
Article type
Communication
Submitted
04 Sep 2000
Accepted
03 Oct 2000
First published
23 Oct 2000

Analyst, 2000,125, 1905-1907

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A fluorescent-labeled oligopeptide for monitoring PKA-mediated phosphorylation

Y. Ohuchi, Y. Katayama and M. Maeda, Analyst, 2000, 125, 1905 DOI: 10.1039/B007152H

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