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Issue 11, 2000
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Determination of 4-nonylphenol and 4-octylphenol in human blood samples by high-performance liquid chromatography with multi-electrode electrochemical coulometric-array detection

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Abstract

Alkylphenols can affect human health because they disrupt the endocrine system. In this study, an analytical method for determining trace amounts of 4-nonylphenol (NP) and 4-octylphenol (OP) in human blood samples was developed. Reversed-phase HPLC with multi-electrode electrochemical coulometric-array detection was used for the determination of NP and OP in plasma and serum samples prepared with a solid-phase extraction method. The separation was achieved using an isocratic mobile phase of 0.7% phosphoric acidacetonitrile with a C18 reversed phase column. The detection limits of NP and OP were 1.0 and 0.5 ng ml−1, respectively. The recoveries of NP and OP added to human plasma samples were above 70.0% with a relative standard deviation of less than 15.5%. The method was found to be applicable to the determination of NP and OP in various human blood samples such as serum and plasma.

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Publication details

The article was received on 11 Aug 2000, accepted on 14 Sep 2000 and first published on 25 Oct 2000


Article type: Paper
DOI: 10.1039/B006597H
Analyst, 2000,125, 1959-1961

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    Determination of 4-nonylphenol and 4-octylphenol in human blood samples by high-performance liquid chromatography with multi-electrode electrochemical coulometric-array detection

    K. Inoue, Y. Yoshimura, T. Makino and H. Nakazawa, Analyst, 2000, 125, 1959
    DOI: 10.1039/B006597H

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