A multi-residue method has been developed for the quantitative
determination of moxidectin, abamectin, doramectin and ivermectin in liver
samples, with capability for qualitative identification of the presence of
eprinomectin. Liver samples are extracted with isooctane, followed by
clean-up on alumina-N solid phase extraction (SPE) cartridges. Extracts are
derivatised and determined by high-performance liquid chromatography (HPLC)
with fluorescence detection. The method was validated using bovine liver
fortified at levels of 4 and 20 μg kg−1 with the drugs.
The mean recovery from bovine liver ranged between 90 and 96%. The intra
and inter-assay variations showed RSD typically of <5% and <10%,
respectively. The procedure was applied also to ovine and porcine liver,
giving similar results. A robustness study, carried out on the alumina
clean-up step, indicated that the step is relatively insensitive to method
changes. However, significant differences overall were found for the type
of alumina and/or commercial SPE cartridge used. The limit of quantitation
of the method is 2 μg kg−1 (ppb).
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