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Issue 3, 2000
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HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas

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Abstract

A simple and fast high performance liquid chromatographic method for five catechins and caffeine using an ODS column and a water–acetonitrile–formic acid mobile phase system was developed. The catechins (epicatechin, catechin, epigallocatechin, epigallocatechin gallate, epicatechin gallate) and caffeine were separated by an acetonitrile gradient within 20 min. The detection limit of the method was approximately 10 ng for all the compounds (by injecting 10 μL). Several green, black and instant teas were analysed using this method. By using the studied compounds as chemical descriptors, linear discriminant analysis was performed and complete differentiation of the green, black and instant teas was achieved.

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Article information


Submitted
22 Nov 1999
Accepted
05 Jan 2000
First published
07 Feb 2000

Analyst, 2000,125, 421-425
Article type
Paper

HPLC determination of catechins and caffeine in tea. Differentiation of green, black and instant teas

P. L. Fernández, M. J. Martín, A. G. González and F. Pablos, Analyst, 2000, 125, 421
DOI: 10.1039/A909219F

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