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Issue 4, 2000
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Direct determination of t,t-muconic acid in human urine by two-dimensional liquid chromatography

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Abstract

The accurate determination of E,E-2,4-hexadiendioic acid (t,t-muconic acid), an important urinary biomarker of benzene exposure, is directly performed in human urine by a new two-dimensional liquid chromatographic method. After a first partial separation of urine components by a reversed-phase mechanism and the focalisation of the anionic analytes on a small anion-chromatographic column, a conventional ion-chromatographic analysis with UV detection is carried out. The analytical procedure is fully controlled by the HPLC instrumentation software using an eight-port switch valve. If compared with conventional one-dimensional procedures, the new method produces chromatograms containing a limited number of well resolved peaks and consequently allows better analytical performances: no interfering peaks, absence of bias, repeatability better than 5% in the concentration range 0.09–5 mg dm−3 and a detection limit of 4.0 μg dm−3 (α = β = 0.05) for the analysis of real samples.

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Publication details

The article was received on 13 Jan 2000, accepted on 10 Feb 2000 and first published on 21 Mar 2000


Article type: Paper
DOI: 10.1039/B000349M
Analyst, 2000,125, 689-692

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    Direct determination of t,t-muconic acid in human urine by two-dimensional liquid chromatography

    P. Serena, A. Tapparo and G. G. Bombi, Analyst, 2000, 125, 689
    DOI: 10.1039/B000349M

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